Current Protocols

UNIT 4.7: Cryo-Immunogold Electron Microscopy

Video 1. Preparing a stainless steel loop for manipulation of grids
Launch Video (6.45 MB)

Video 2. Labeling and washing grid-mounted sections
Launch Video (3.78 MB)

Video 3. Methylcellulose embedding
Launch Video (2.55 MB)

Video 4. Fixation of cells for cryo-immunogold labeling
Launch Video (5.70 MB)

Video 5. Sucrose infiltration tissues/cells fixed for cryo-immunogold labeling
Launch Video (9.77 MB)

Video 6. Plunge freezing
Launch Video (5.96 MB)

Video 7. Preparing a mounted eyelash for handling sections
Launch Video (3.81 MB)

Video 8. Ultrathin cryo-sectioning
Launch Video (9.15 MB)

Video 9. Picking up sections after ultrathin sectioning
Launch Video (1.49 MB)

Video 10. Ultrathin section retrieval and transfer to Formvar-coated grids
Launch Video (9.08 MB)

Video 11. Coating grids with Formvar
Launch Video (11.14 MB)

UNIT 4.16: Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis

Video 1. Tubule formation on a vesicle during endocytosis.
Launch Video (1.58 MB)

UNIT 22.9: Assembly and Micromanipulation of Xenopus In Vitro–Assembled Mitotic Chromosomes

Video 1. Chromosomal stretching experiment using a Xenopus in vitro–assembled mitotic chromosome (Introduction/Basic Protocol)
Launch Video (2.15 MB)

Video 2. Measuring the spring constant of a micropipet (Support Protocol 6)
Launch Video (1.17 MB)

Video 3. Calibration of the needle with masses (Support Protocol 7)
Launch Video (1.23 MB)