Current Protocols

Computational Analysis.

Detection and Assay Methods.

Extraction, Stabilization, and Concentration.

Production of Recombinant Proteins.

Purification of Recombinant Proteins.

Characterization of Recombinant Proteins.

Conventional Chromatographic Separations.

Affinity Purification.

Electrophoresis.

Chemical Analysis.

Post-Translational Modification: Glycosylation.

Post-Translational Modification: Phosphorylation and Phosphatases.

Post-Translational Modification: Specialized Applications.

Chemical Modification of Proteins.

Mass Spectrometry.

Preparation and Handling of Peptides.

Identification of Protein Interactions.

Quantitation of Protein Interactions.

Peptidases.

Appendix 1: Reagents and Solutions.

Appendix 2: Useful Measurements.

Appendix 3: Commonly Used Techinques.

Appendix 4: Suppliers.

References.