Current Protocols

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What's New and Updated in Current Protocols in Molecular Biology
Supplement 82, April 2008

UNIT 13.2 Growth and Manipulation of Yeast (Updated)
UNIT 13.3B EMS and UV Mutagenesis in Yeast (New)
UNIT 14.8 In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets (Updated)
UNIT 14.17 Using CellProfiler for Automatic Identification and Measurement of Biological Objects in Images (New)
UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins (Updated)
UNIT 23.5 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout) (Updated)
UNIT 23.6 Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout) (Updated)

An essential tool for anyone at the forefront of today's molecular biology revolution, Current Protocols in Molecular Biology --the first Current Protocols title--remains the benchmark by which all other lab manuals are judged.

Updated every three months in all formats, Current Protocols in Molecular Biology (CPMB) is constantly evolving to keep pace with the very latest discoveries and developments. The entire collection of published methods plus a year of these quarterly updates are included in the initial purchase price. That's 4900 published pages of content amassed since 1987, plus 670 pages of new (39%) and revised (59%) content over the course of the year! Presently five volumes in its loose-leaf print version, Current Protocols in Molecular Biology ...

• includes more than 1,000 procedures from hundreds of biologists at top laboratories around the globe.
• offers a range of basic methods for DNA preparation to advanced procedures for microarray analysis, chromatin assembly, and more.
• covers emerging areas, including mouse phenotyping, DNA construction by recombination, and high-throughput sequencing.

Edited by: Fred M. Ausubel (Massachusetts General Hospital and Harvard Medical School); Roger Brent (The Molecular Sciences Institute); Robert E. Kingston (Massachusetts General Hospital and Harvard Medical School); David D. Moore (Baylor College of Medicine); J.G. Seidman (Harvard Medical School); John A. Smith (University of Alabama at Birmingham); Kevin Struhl (Harvard Medical School)

Developmental Editor: Elizabeth W. Harkins

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.