The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses

Gil Stelzer1, Naomi Rosen1, Inbar Plaschkes2, Shahar Zimmerman3, Michal Twik3, Simon Fishilevich3, Tsippi Iny Stein3, Ron Nudel3, Iris Lieder2, Yaron Mazor2, Sergey Kaplan2, Dvir Dahary4, David Warshawsky5, Yaron Guan‐Golan5, Asher Kohn5, Noa Rappaport3, Marilyn Safran3, Doron Lancet6

1 These authors contributed equally to the paper, 2 LifeMap Sciences Ltd, Tel Aviv, 3 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 4 Toldot Genetics Ltd, Hod Hasharon, 5 LifeMap Sciences Inc, Marshfield, Massachusetts, 6 Corresponding author
Publication Name:  Current Protocols in Bioinformatics
Unit Number:  Unit 1.30
DOI:  10.1002/cpbi.5
Online Posting Date:  June, 2016
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GeneCards, the human gene compendium, enables researchers to effectively navigate and inter‐relate the wide universe of human genes, diseases, variants, proteins, cells, and biological pathways. Our recently launched Version 4 has a revamped infrastructure facilitating faster data updates, better‐targeted data queries, and friendlier user experience. It also provides a stronger foundation for the GeneCards suite of companion databases and analysis tools. Improved data unification includes gene‐disease links via MalaCards and merged biological pathways via PathCards, as well as drug information and proteome expression. VarElect, another suite member, is a phenotype prioritizer for next‐generation sequencing, leveraging the GeneCards and MalaCards knowledgebase. It automatically infers direct and indirect scored associations between hundreds or even thousands of variant‐containing genes and disease phenotype terms. VarElect's capabilities, either independently or within TGex, our comprehensive variant analysis pipeline, help prepare for the challenge of clinical projects that involve thousands of exome/genome NGS analyses. © 2016 by John Wiley & Sons, Inc.

Keywords: biological database; bioinformatics; diseases; GeneCards; gene prioritization; human genes; integrated information retrieval; next generation sequencing; VarElect

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Table of Contents

  • Introduction
  • Basic Protocol 1: Searching and Browsing GeneCards
  • Basic Protocol 2: Exploring a GeneCard
  • Basic Protocol 3: Using VarElect
  • Accessibility
  • Guidelines for Understanding Results
  • Commentary
  • Literature Cited
  • Figures
  • Tables
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Basic Protocol 1: Searching and Browsing GeneCards

  Necessary Resources
  • An up‐to‐date Web browser such as Google Chrome, Mozilla Firefox, Microsoft Edge, or Apple Safari

Basic Protocol 2: Exploring a GeneCard

  Necessary Resources
  • An up‐to‐date Web browser such as Google Chrome, Mozilla Firefox, Microsoft Edge, or Apple Safari

Basic Protocol 3: Using VarElect

  Necessary Resources
  • An up‐to‐date Web browser such as Google Chrome, Mozilla Firefox, Microsoft Edge, or Apple Safari
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Internet Resources
  GeneCards is a compendium of human genes that provides genomic, proteomic, transcriptomic, genetic, and functional information on all known and predicted human genes. It is developed and maintained by the Lancet lab in the Department of Molecular Genetics at the Weizmann Institute of Science.
  VarElect is a cutting‐edge Variant Election application for disease/phenotype‐dependent gene variant prioritization. It is an effective and user friendly tool for analyzing genes with variants following Next‐Generation Sequencing (“NGS”) experiments. VarElect can rapidly prioritize genes that have been found to have variants according to selected disease/phenotype‐gene associations.
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