Obtaining Comparative Genomic Data with the VISTA Family of Computational Tools

Igor Ratnere1, Inna Dubchak1

1 Lawrence Berkeley National Laboratory, Berkeley, California
Publication Name:  Current Protocols in Bioinformatics
Unit Number:  Unit 10.6
DOI:  10.1002/0471250953.bi1006s26
Online Posting Date:  June, 2009
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Comparison of DNA sequences from different species is a fundamental method for identifying functional elements, such as exons or enhancers, as they tend to exhibit significant sequence similarity due to purifying selection. Availability of whole‐genome sequences for a constantly growing number of organisms makes identification of such elements within these genomes possible. There are two distinct phases in comparisons of genomic sequences: in the first, the sequences are aligned, and in the second, the resulting alignments are analyzed to find conservation signals that may be indicative of functional regions. Due to the considerable length of alignments, good visual representation techniques are a necessity for effective isolation of regions of interest. The VISTA family of tools provides biomedical investigators with a unified framework for the alignment of long genomic sequences and whole‐genome assemblies, interactive visual analysis of alignments along with functional annotation, and many other comparative genomics capabilities. Curr. Protoc. Bioinform. 26:10.6.1‐10.6.17. © 2009 by John Wiley & Sons, Inc.

Keywords: comparative genomics; DNA alignment; VISTA; genome browser

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Table of Contents

  • Introduction
  • Basic Protocol 1: Analyzing Comparative Genomic Data with the VISTA Browser
  • Basic Protocol 2: Browsing the Alignment and Retrieving SNP Information Using Base‐Pair Level Alignment Panel
  • Basic Protocol 3: Obtaining Detailed Comparative Data Including Genomic Coordinates and Parameters of Conserved Regions with the Text Browser
  • Basic Protocol 4: Finding Candidate Orthologous Regions on a Base Genome Using the GenomeVISTA Server
  • Basic Protocol 5: Finding Putative Transcription Factor Binding Sites (TFBS) Using rVISTA Server
  • Basic Protocol 6: Finding Experimentally Verified Enhancers Using the VISTA Enhancer Browser
  • Understanding Results
  • Commentary
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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