Media and Culture of Yeast

Simon R. Green1, Charles M. Moehle2

1 Fluorescience Ltd., Leeds, 2 Genelabs Technologies, Inc., Redwood City, California
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 1.6
DOI:  10.1002/0471143030.cb0106s04
Online Posting Date:  May, 2001
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Abstract

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast.

     
 
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Table of Contents

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Materials

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Literature Cited

Key References
   Guthrie, C. and Fink, G.R. (eds.) 1991. Guide to yeast genetics and molecular biology. Methods Enzymol. vol. 194.
  A compendium of technical methods in yeast cell culture, genetics, biochemistry, molecular biology, and cell biology.
   Rose, M.D., Winston, F., and Hieter, P. 1990. Methods in Yeast Genetics, A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  The laboratory manual from a Cold Spring Harbor laboratory source on yeast molecular biology.
Internet Resources
   http://www.atcc.org/
  Web site for the ATCC.
   http://www.atcc.org/hilights/sc_info.html
  Web site for the ATCC Saccharomyces page.
   http://dgm2ibm.nihs.go.jp/ygsc.htm
  This Web page provides access to YGSC catalogs, though it is not official.
   http://genome‐www.stanford.edu/Saccharomyces/
  This Web site contains an extensive amount of information and links related to Saccharomyces.
   http://www.tiac.net/users/cstyles/
  This Web site contains specific information about Saccharomyces and is a source for some harder‐to‐find equipment.
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