Polarization Microscopy

Shinya Inoué1

1 Marine Biological Laboratory, Woods Hole, Massachusetts
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 4.9
DOI:  10.1002/0471143030.cb0409s13
Online Posting Date:  February, 2002
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This unit provides an introduction to polarization microscopy, the optics involved, and practical considerations for observing submicroscopic structures. The unit also includes specific examples for visualization of microtubules in the mitotic spindle, chromatin within maturing spermatids, and the biocrystalline skeletal spicules in larval echinoderms.

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Table of Contents

  • Introduction
  • Color in Polarized Light
  • Optical Rotation
  • Video Microscopy
  • Applications of Polarization Microscopy
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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