Digital Electrophoresis Analysis

Scott Medberry1, Sean Gallagher2

1 Amersham Pharmacia Biotech, San Francisco, California, 2 Motorola Corporation, Tempe, Arizona
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 6.9
DOI:  10.1002/0471143030.cb0609s16
Online Posting Date:  November, 2002
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Abstract

Digital recording of gel images offers several advantages over conventional photography. Capture of the image is quick and reliable, retaining the image in a medium that allows digital analysis of the image. These images are easy to handle, accurate, reproducible and less expensive to generate. This unit provides a guide to digital capture and analysis, discussing the equipment and methods for image capture and the process of image analysis for one‐ and two‐dimensional gels.

     
 
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Table of Contents

  • Reasons for Digital Documentation and Analysis
  • Key Terms for Imaging
  • Image Capture
  • Analysis
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Appel, R.D., Hochstrasser, D.F., Funk, M., Vargas, J.R., Muller, A.F., and Scherrer, J.‐R. 1991. The MELANIE project: From a biopsy to automatic protein map interpretation by computer. Electrophoresis 12:722‐735.
   Garrels, J.I., 1989. The QUEST system for quantitative analysis of two‐dimensional gels. J. Biol. Chem. 264:5269‐5282.
   Glasbey, C.A. and Horgan, G.W., 1994. Image Analysis for the Biological Sciences. John Wiley & Sons, Chichester, England.
   Hamming, R.W., 1973. Numerical methods for scientists and engineers, 2nd ed. Dover Publications, New York.
   Huffman, D.A. 1952. A method for the construction of minimum‐redundancy codes. Proc. Inst. Elect. Radio Eng. 40:9‐12.
   Monardo, P.J., Boutell, T., Garrels, J.I., and Latter, G.I. 1994. A distributed system for two‐dimensional gel analysis. Comput. Appl. Biosci. 10:137‐143.
   Patton, W.F. 1995. Biologist's perspective on analytical imaging systems as applied to protein gel electrophoresis. J. Chromatogr. A. 698:55‐87.
   Plikaytis, B.D., Carlone, G.M., Edmonds, P., and Mayer, L.W. 1986. Robust estimation of standard curves for protein molecular weight and linear‐duplex DNA base‐pair number after gel electrophoresis. Anal. Biochem. 152:346‐364.
   Russ, J.C. 1995. The Image Processing Handbook. CRC Press, Boca Raton, Fla.
   Smith, J.M. and Thomas, D.J. 1990. Quantitative analysis of one‐dimensional gel electrophoresis profiles. Comput. Appl. Biosci. 6:93‐99.
   Sutherland, J.C., Lin, B., Monteleone, D.C., Mugavero, J., Sutherland, B.M., and Trunk, J. 1987. Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: Application to ethidium bromide–stained DNA. Anal. Biochem. 163:446‐457.
   Welch, T.A. 1984. A technique for high performance data compression. IEEE Computer. 17:21‐32.
Key References
   Glasbey and Horgan, 1994. See above.
  Describes general image‐processing techniques as they are applied to biological images.
   Russ 1995. See
  A general reference book on digital image capture and analysis.
   Sutherland, J.C. 1993. Electronic imaging of electrophoretic gels and blots. In Advances in Electrophoresis, Vol. 6. (A. Chrambach, M.J. Dunn, and B.J. Radola, eds.) pp. 1‐41. VCH Verlagsgesellschaft mbH, Weinheim, Germany.
  Provides an overview of image capture with particular emphasis on types of capture equipment.
Internet Resources
  rsb.info.nih.gov/nih‐image
  NIH Image is free software that provides basic image analysis tools for the Macintosh.
  http://www.inforamp.net/∼poynton/Poynton‐color.html
  Contains an excellent description of gamma correction in the Gamma FAQ.
  http://www‐Immb.ncifcrf.gov/EP/table2Ddatabases.html
  A list of links to many two‐dimensional databases that are available via the Internet.
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