Overview of Eukaryotic In Vitro Translation and Expression Systems

Rosemary Jagus1, Gregory S. Beckler2

1 Center of Marine and Biotechnology and Greenebaum Cancer Center, Baltimore, Maryland, 2 Promega Corporation, Madison, Wisconsin
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 11.1
DOI:  10.1002/0471143030.cb1101s00
Online Posting Date:  April, 2003
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The ability to investigate cellular processes in vitro permits detailed analysis of the process and its molecular components. Eukaryotic translation and expression is one system that has been well studied. This overview describes the development of in vitro systems, including such approaches as continuous‐flow systems, coupled transcription/translation, and the incorporation of non‐natural amino acids. It also discusses molecular and genetic studies to probe translation, including post‐translational fate of the synthesized proteins.

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Table of Contents

  • Early History
  • Improved Systems
  • Newer Systems and Approaches
  • New Applications
  • Molecular Function
  • The Future of In Vitro Translation Systems
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Internet Resources
  Bibliography of references using the TNT coupled trancription/translation system, compiled by G.S. Beckler, 1998. Promega Corp., Madison, Wis.
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