Overview: Engineering Transgenic Constructs and Mice
Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mouse models are now considered essential to correctly understand disease processes in whole animals. This unit reviews the basic mouse technologies used to generate conventional transgenic mice, which represent a gain-of-function approach. First, an overview of transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques. It then describes typical problems that are encountered when researchers start to generate transgenic mice. Curr. Protoc. Cell Biol. 42:19.10.1-19.10.9. © 2009 by John Wiley & Sons, Inc.
Keywords: transgenic mice; plasmid vector; constructs; gene expression; reporter gene
Table of Contents
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|Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2003. "Production of transgenic mouse". In Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed. pp. 289-358. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.|
This book is one of the most read laboratory manuals for the production of transgenic mice.
|Voncken, J.W. 2003. "Genetic modification of the mouse: General technology; pronuclear and blastocyst injection". In Transgenic Mouse: Methods and Protocols (M.H. Hofker and J. van Deursen, eds.) pp. 9-34. Humana Press, Totowa, N. J.|
General mouse technologies including basic husbandry, and microinjections are very well described.
PubMed is the world's largest literature database. Search using keywords transgenic, mouse, and the tissue or cell type of interest to find out the preferable promoters.
Nagy Lab Cre and Flox mouse database.
International Mouse Strain Resource.
Mouse genome informatics.
These Jackson Laboratory website provide links to a variety of mouse-related information, such as mutant resources and literature pertaining to mouse genetics.