Optimization of Transfection

John K. Rose1

1 Yale University School of Medicine, New Haven, Connecticut
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 20.7
DOI:  10.1002/0471143030.cb2007s19
Online Posting Date:  August, 2003
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Abstract

When embarking upon any transfection procedure, a critical first step is to optimize conditions. Every mammalian cell type has a characteristic set of requirements for optimal introduction of foreign DNA; there is a tremendous degree of variability in the transfection conditions that work, even among cell types that are very similar to one another. Often, an experimenter must screen a wide variety of cell types for a desired regulatory trait, such as an appropriate response to a particular effector molecule. It is thus helpful to have a straightforward, systematic approach to optimizing transfection efficiency. This unit provides guidelines to optimizing transfection experiments.

     
 
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Table of Contents

  • Calcium Phosphate Transfection
  • DEAE‐Dextran Transfection
  • Electroporation
  • Liposome‐Mediated Transfection
  • Tables
     
 
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Materials

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Literature Cited

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