Design and Use of Fluorescent Fusion Proteins in Cell Biology

Erik Snapp1

1 Albert Einstein College of Medicine, Bronx, New York
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 21.4
DOI:  10.1002/0471143030.cb2104s27
Online Posting Date:  July, 2005
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Abstract

This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.

Keywords: live cell imaging; GFP; photoactivation; RFP; ReAsH; signal sequence; biarsenical tetracysteine

     
 
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Table of Contents

  • Basic Protocol 1: Design of a Fluorescent Fusion Protein
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

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Literature Cited

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