Photoactivation and Imaging of Photoactivatable Fluorescent Proteins

George H. Patterson1

1 Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Unit 21.6
DOI:  10.1002/0471143030.cb2106s38
Online Posting Date:  March, 2008
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A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be “turned on” by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks. Curr. Protoc. Cell Biol. 38:21.6.1‐21.6.10. © 2008 by John Wiley & Sons, Inc.

Keywords: photoactivatable; fluorescent protein; microscopy

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Table of Contents

  • Introduction
  • Background
  • Requirements for Photoactivating Fluorescent Proteins
  • Optimization Procedures
  • General Photoactivation Experiment
  • Uses of Photoactivatable Fluorescent Proteins
  • Future Directions of PA‐FPs
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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