Centrifuges and Rotors


Publication Name:  Current Protocols in Cell Biology
Unit Number:  Appendix 1G
DOI:  10.1002/0471143030.cba01gs07
Online Posting Date:  May, 2001
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Abstract

This appendix lists rmax values (maximum value of the rotating radius between the particle being centrifuged and the axis of rotation) for commonly used rotors manufactured by Sorvall, Beckman Coulter, Fisher, and IEC. In addition, nomograms are provided for the determination of the relative centrifugal force (RCF) where speed and rmax are known, or the speed where RCF and rmax are known. This is done by aligning a ruler across the two known values and reading the unknown value at the point where the ruler crosses the remaining column. Also discussed are the characteristics of the various materials used to construct centrifuge tubes, including optical properties, appropriate methods for sterilization, and chemical resistances (tolerance to various media, organic solvents, and alcohols).

     
 
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Table of Contents

  • Internet Resources
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Internet Resources
   http://www.sorvall.com
  A userful site from Sorvall for obtaining product information about centrifuges and rotors; includes a site for a downloadable RCF calculator.
   http://www.beckmancoulter.com/beckman/biorsch/prodinfo/cntrifug/centuser.asp
  Contains Beckman centrifuge product information and sites for computing RCF, speed, and k‐factor.
   http:://www.fishersci.com
  Catalog site for information about centrifuges produced by Clay Adams, Eppendorf, FiberLite, Fisher, and IEC.
  http://labcentrifuge.com
  Catalog site for information about IEC centrifuges and rotors.
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