Common Stock Solutions, Buffers, and Media


Publication Name:  Current Protocols in Cell Biology
Unit Number:  Appendix 2A
DOI:  10.1002/0471143030.cba02as00
Online Posting Date:  May, 2001
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Abstract

This section describes the preparation of buffers and reagents used in this manual for cell culture, manipulation of tissue, and cell biological methods. Also discussed are special considerations for PCR experiments and for working with RNA.

    Recipes

  • Acid, concentrated stock solutions

  • Acid precipitation solution

  • Ammonium hydroxide, concentrated stock solution

  • Ammonium acetate, 10 M

  • Ammonium sulfate, saturated

  • ATP, 100 mM

  • Base, concentrated stock solutions

  • BSA (bovine serum albumin), 10% (w/v)

  • CaCl2, 1 M

  • Carbonate buffer

  • CMF‐DPBS (calcium‐ and magnesium‐free Dulbecco's phosphate‐buffered saline)

  • DEPC (diethylpyrocarbonate)‐treated solutions

  • DMEM (Dulbecco's modified Eagle medium), supplemented

  • DPBS (Dulbecco's phosphate‐buffered saline)

  • DTT (dithiothreitol), 1 M

  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)

  • FBS (fetal bovine serum)

  • HBSS (Hanks' balanced salt solution)

  • HCl, 1 M

  • HeBS (HEPES‐buffered saline) solution, 2×

  • KCl, 1 M

  • LB medium

  • MgCl2, 1 M

  • MgSO4, 1 M

  • NaCl, 5 M

  • NaOH, 10 M

  • PBS (phosphate‐buffered saline)

  • PCR amplification buffer, 10×

  • PMSF (phenylmethylsulfonyl fluoride), 100 mM

  • Polylysine‐coated tissue culture surfaces

  • Potassium acetate buffer, 0.1 M

  • Potassium phosphate buffer, 0.1 M

  • Saponin, 10% (w/v)

  • SDS, 20% (w/v)

  • SDS electrophoresis buffer, 5×

  • SDS sample buffer

  • Sodium acetate, 3 M

  • Sodium acetate buffer, 0.1 M

  • Sodium phosphate buffer, 0.1 M

  • TAE (Tris/acetate/EDTA) electrophoresis buffer, 10×

  • TBE (Tris/borate/EDTA) electrophoresis buffer, 10×

  • TBS (Tris‐buffered saline)

  • TE (Tris/EDTA) buffer

  • TEA (triethanolamine) solution

  • TEN (Tris/EDTA/NaCl) solution

  • Tris⋅Cl, 1 M

  • Triton X‐100, 10% (w/v)

  • TTBS (Tween 20/TBS)

     
 
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Table of Contents

  • Recipes
  • Special Considerations for PCR Experiments
  • Special Consideration for Working with RNA
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
  Chemical Rubber Company. 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data, 3d ed., Vol.1. CRC Press, Boca Raton, Fla.
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