Silanizing Glassware

Brian Seed1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Cell Biology
Unit Number:  Appendix 3E
DOI:  10.1002/0471143030.cba03es08
Online Posting Date:  May, 2001
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Abstract

This unit provides a protocol for silanizing (siliconizing) glassware to prevent adsorption of solute to the glass surface and to increase the hydrophobicity of the surface. This is particularly important when dealing with low concentrations of particularly “sticky” solutes such as single‐stranded nucleic acids or proteins or cells.

     
 
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Table of Contents

  • Commentary
     
 
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Materials

Basic Protocol 1:

  Materials
  • Chlorotrimethylsilane or dichlorodimethylsilane
  • Vacuum pump
  • Desiccator, equipped with a valve
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Figures

Videos

Literature Cited

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