Overview of Flow Cytometry Instrumentation

Phillip N. Dean1, Robert A. Hoffman2

1 null, Livermore, California, 2 BD Biosciences, San Jose, California
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.1
DOI:  10.1002/0471142956.cy0101s39
Online Posting Date:  January, 2007
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Abstract

This unit describes the technology in general, to give a feel for the interplay between the various parts of a flow cytometer. Topics include cell preparation, detectors, analysis, and sorting. A brief chronology of flow instrumentation development illustrates the long history of the field.

     
 
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Table of Contents

  • Cell Preparation
  • Flow Chamber
  • Detectors
  • Analysis
  • Sorting
  • Chronology of Flow Cytometry Development
  • Summary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Melamed, M.R., Lindmo, T., and Mendelsohn, M.L. (eds.) 1990. Flow Cytometry and Cell Sorting, 2nd ed. Wiley‐Liss, New York.
   Shapiro, H.M. 2003. Practical Flow Cytometry, 4th ed. John Wiley & Sons, Hoboken, N.J.
Key References
   Melamed, M.R., Mullaney, P.F., and Mendelsohn, M.L. (eds.) 1979. Flow Cytometry and Cell Sorting, 1st ed. John Wiley & Sons New York.
  First overall summary of the field, with many authors describing the state of the art as of 1979. Covers applications of the technology as well as instrumentation.
   Melamed, Lindmo, and Mendelsohn (eds.) 1990. See above.
  Second edition with all‐new papers, producing true update of the first edition. Also contains extensive history of the field.
   Shapiro, H.M. 2003. See above.
  Comprises user's reference manual for the laboratory. Also includes extensive list of current literature and list of key suppliers of instruments, parts, and reagents.
   Van Dilla, M.A., Dean, P.N., Laerum, O.D., and Melamed, M.R. 1985. Flow Cytometry: Instrumentation and Data Analysis. Academic Press, London.
  Contains papers by leading experts in the field on both subjects.
   Watson, J.U. 2004. Introduction to Flow Cytometry. Cambridge University Press, Cambridge.
  Includes detailed discussion of all aspects of flow cytometry instrumentation and measurement phyics.
Internet Resources
   http://www.isac‐net.org
  Homepage of the International Society for Analytical Cytology (ISAC).
   http://www.cyto.purdue.edu
  Comprehensive cytometry resource.
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