Optical Filter Sets for Multiparameter Flow Cytometry

Alan Waggoner1

1 Amersham Life Sciences, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.5
DOI:  10.1002/0471142956.cy0105s00
Online Posting Date:  May, 2001
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Abstract

One of the most important components of a fluorescence detection system, the optical filter set selects wavelengths of light for fluorophore excitation and fluorescent signal discrimination. Of the variety of optical devices available for wavelength selection, optical filters have gained wide acceptance because of their wavelength selectivity, ease of installation, and relative inexpensiveness. This unit discusses the properties, compositions, and uses of optical filters in flow cytometry. The information should allow investigators to select quality filters for modifying systems for new dyes and multicolor analyses.

     
 
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Table of Contents

  • Spectral Characteristics of Optical Filters
  • Optical Filter Systems for Flow Cytometry
  • Construction of Optical Filters
  • Characteristics of Good Optical Filter Systems for Flow Cytometry
  • Conclusions and Future
  • Acknowledgement
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Aubin, J. 1984. Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27:36‐43.
   Buican, T.N. 1990 Real‐time Fourier transform spectroscopy of fluorescence imaging and flow cytometry. SPIE Proc. 1250:126‐133.
   Farkas, D.L., Ballou, B.T., Fisher, G.W., Fishman, D., Garini, Y., Niu, W., and Wachaman, E.S. 1996. Microscopic and mesoscopic spectral bio‐imaging. SPIE Proc. 2687:200‐209.
   Galbraith, W., Ernst, L.A., Taylor, D.L., and Waggoner, A.S. 1989. Multiparameter fluorescence and selection of optimal filter sets: Mathematics and computer program. SPIE Proc. 1063:74‐122.
   Shapiro, H. 1995. Practical Flow Cytometry, 3rd ed. Wiley‐Liss, New York.
   Waggoner, A.S., Ernst, L.A., Chen, C.H., and Rechtenwald, D.J. 1993. PE‐Cy5: A new fluorescent antibody label for 3‐color flow cytometry with a single laser. Ann. N.Y. Acad. Sci. 667:185‐193.
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