Principles of Gating
Few aspects of flow cytometry are common to every application. Gating principles is one. This unit provides an excellent overview as well as detailed conceptual material on how gating works, when certain types of gating should or should not be applied, and the advantages and disadvantages of various techniques. This unit should be required reading for every technician using a flow cytometer and every graduate student starting out in the area, and is an excellent refresher for those more advanced in the technology. It brings out the historical development of our understanding of gating applications as well as providing a high level of technical accuracy.
Figure 1.8.3 Light-scatter gating is commonly used to identify lymphocytes, monocytes, and granulocytes in a whole blood preparation.
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|Mandy, F.F., Bergeron, M., Recktenwald, D., and Izaguirre, C.A. 1992. A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol: Comparison with conventional two-color immunophenotyping method. J Immunol. Methods 156:151-156.|
|McCoy, J.P. Jr., Chambers, W.H., Lakomy, R., Campbell, J.A., and Stewart, C.C. 1991. Sorting minor subpopulations of cells: Use of fluorescence as the triggering signal. Cytometry 12:268-274.|
|Murphy, R.F. 1985. Automated identification of subpopulations in flow cytometric list mode data using cluster analysis. Cytometry 6:302-309.|
|Nicholson, J.K.A., Jones, B.M., and Hubbard, M. 1993. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry 14:685-689.|
|Redelman, D. 1993. Improved procedures for training neural networks to analyze flow cytometric data. Cytometry (Suppl.)6:43.|
|Snow, C. and Bauer, K. 1994. Usefulness of DNA fluorescence ratioing in reducing DNA measurement artifacts caused by aggregates. Cytometry (Suppl.)7:50.|
|van den Engh, G. and Stokdijk, W. 1989. Parallel processing data acquisition system for multilaser flow cytometry and cell sorting. Cytometry 10:289-293.|
|Shapiro, H.M. 1995. Practical Flow Cytometry, 3rd ed. Wiley-Liss, New York.|
A resource for more examples of the use of gating in flow cytometry applications.