Techniques for Flow Cytometer Alignment

James H. Jett1, John C. Martin1, Robert C. Habbersett1

1 National Flow Cytometry Resource, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.10
DOI:  10.1002/0471142956.cy0110s50
Online Posting Date:  October, 2009
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Abstract

This unit provides essential knowledge for correctly using any flow cytometer to ensure that data collected are accurate and reliable. Two levels of system alignment are described: routine alignment checks and complete alignment, both of which can be applied to a variety of instrument configurations. Curr. Protoc. Cytom. 50:1.10.1‐1.10.7. © 2009 by John Wiley & Sons, Inc.

Keywords: flow cytometer alignment; laser beam profile analysis; oscilloscope

     
 
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Table of Contents

  • Introduction
  • Verification of Alignment
  • Methods for Complete System Alignment
  • Other Resources
  • Acknowledgements
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

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