Flow Cytometers for Characterization of Microorganisms

Harald B. Steen1

1 Norsk Hydro Institute for Cancer Research, Oslo, Norway
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.11
DOI:  10.1002/0471142956.cy0111s07
Online Posting Date:  May, 2001
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Abstract

The measurement of microbes is an increasingly important application in flow cytometry. The small size of these organisms can create special difficulties for instruments designed to analyze much larger eukaryotic cells. This unit describes the problems encountered in making microbiological measurements, the instrument requirements, and some approaches that can be taken to optimize the instrument. This material is essential background for the protocols found in chapter 11, Microbiological Applications.

     
 
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Table of Contents

  • Flow Cytometer Designs
  • Sensitivity and Resolution
  • Light‐Scattering Detection
  • Practical Considerations
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Bernander, R., Stokke, T., and Boye, E. 1998. Flow cytometry of bacterial cells: Comparison between different flow cytometers and different DNA stains. Cytometry 31:29‐36
   Kachel, V., Fellner‐Feldegg, H., and Menke, E. 1990. Hydrodynamic properties of flow cytometric instruments. In Flow Cytometry and Sorting (M.R. Melamed, T. Lindmo, and M.L. Mendelsohn, eds.) pp. 27‐44 Wiley‐Liss, New York.
   Pinkel, D. and Stovel, R. 1985. Flow chambers and sample handling. Flow Cytometry: Instrumentation and Data Handling (M.A. Van Dilla, P.N. Dean, O.D. Laerum, and M.R. Melamed, eds.) pp. 77‐128 Academic Press, London.
   Steen, H.B., 1986. Simultaneous separate detection of low angle and large angle light scattering in an arc lamp‐based flow cytometer. Cytometry 7:445‐449
   Steen, H.B., 1990. Characteristics of flow cytometers. Flow Cytometry and Sorting (M.R. Melamed, T. Lindmo, and M.L. Mendelsohn, eds.) pp. 11‐25. Wiley‐Liss, New York.
   Steen, H.B. 1992. Noise, sensitivity, and resolution of flow cytometers. Cytometry 13:882‐830
   Steen, H.B. and Lindmo, T. 1979. Flow cytometry: A high resolution instrument for everyone. Science 204:403‐404
   Steen, H.B. and Sorensen, O.I. 1993. Pulse modulation of the excitation light source boosts the sensitivity of an arc lamp‐based flow cytometer. Cytometry 14:115‐122
   Steen, H.B., Jernes, M.W., Skarstad, K., and Boye, E. 1994. Staining and measurement of DNA in bacteria. Methods in Cell Biology, Vol. 42, Part B (Z. Darzynkiewicz, J.P. Robinson, and H.A. Crissman, eds.) pp. 477‐487 Academic Press, San Diego.
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