Principles of Resonance Energy Transfer

János Szöllősi1, Sándor Damjanovich1, Péter Nagy1, György Vereb1, László Mátyus1

1 University Medical School of Debrecen, Debrecen
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.12
DOI:  10.1002/0471142956.cy0112s38
Online Posting Date:  November, 2006
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This unit describes the basic principles of the fluorescence resonance energy (FRET) process. In addition, it characterizes available parameters and instruments for FRET measurements, discusses limitations, and shows a few examples of the application of FRET.

Keywords: fluorescence resonance energy transfer; Förster distance; tandem dyes; cell surface mapping

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Table of Contents

  • Theory of FRET
  • Determination of Förster Distance (R0)
  • How to Measure FRET Efficiency
  • Choice of Instrument for FRET Measurement
  • Limitations of FRET Studies
  • Applications
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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