Plug Flow Cytometry

Bruce S. Edwards1, Larry A. Sklar2

1 University of New Mexico, Albuquerque, New Mexico, 2 Los Alamos National Laboratory, National Flow Cytometry Resource, Los Alamos, New Mexico
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.17
DOI:  10.1002/0471142956.cy0117s17
Online Posting Date:  August, 2001
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Abstract

Although flow cytometry has the powerful ability to rapidly screen large collections of cells, the technology has yet to be efficiently applied to large‐scale screening operations involving multiple discrete suspensions. High‐throughput flow cytometry would be beneficial to many areas of biological investigation, such as modern drug discovery, which involves testing of cellular targets against millions of potentially valuable compounds. The authors have developed a flow injection analysis approach to automated sample handling in which individual sample suspensions are sequentially inserted as plugs of precisely defined volumes into a flowing fluid which delivers them to the laser beam. The unit describes the basic elements and concepts of this plug flow system and discusses representative applications.

     
 
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Table of Contents

  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Edwards, B.S., Kuckuck, F., and Sklar, L.A. 1999. Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis. Cytometry 37:156‐159.
   Edwards, B.S., Curry, M.S., Tsuji, H., Larson, R.S., Brown, D., and Sklar, L.A. 2000. Expression of P‐selectin at low site density promotes selective recruitment of eosinophils over neutrophils. J. Immunol. 165:404‐410.
   Edwards, B.S., Kuckuck, F.W., Prossnitz, E.R., Ransom, J.T., and Sklar, L.A. 2001a. HTPS flow cytometry: A novel platform for automated high throughput drug discovery and characterization. J. Biomol. Screening 6:83‐90.
   Edwards, B.S., Kuckuck, F.W., Prossnitz, E.R., Okun, A., Ransom, J.T., and Sklar, L.A. 2001b. Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology. Cytometry 43:211‐216.
   Kachel, V., Fellner‐Feldegg, H., and Menke, E. 1990. Hydrodynamic properties of flow cytometry instruments. In Flow Cytometry and Sorting, 2nd ed. (M.R. Melamed, T. Lindmo, and M.L. Mendelsohn, eds.) pp. 27‐45. Wiley‐Liss, New York.
   Lindberg, W., Ruzicka, J., and Christian, G.D. 1993. Flow injection flow cytometry: A new approach for sample and solution handling in flow cytometry. Cytometry 14:230‐236.
   Nolan, J.P. and Sklar, L.A. 1998. The emergence of flow cytometry for sensitive, real‐time measurements of molecular interactions. Nature Biotechnology 16:633‐638.
   Nolan, J.P., Lauer, S., Prossnitz, E.R., and Sklar, L.A. 1999. Flow cytometry: A versatile tool for all phases of drug discovery. Drug Discov. Today 4:173‐180.
   Seamer, L.C., Kuckuck, F., and Sklar, L.A. 1999. Sheath fluid control to permit stable flow in rapid mix flow cytometry. Cytometry 35:75‐79.
   Sklar, L.A., Seamer, L.C., Kuckuck, F., Posner, R., Prossnitz, E., Edwards, B., and Nolan, J.P. 1998. Sample handling for kinetics and molecular assembly in flow cytometry. S.P.I.E. Proc. 3256:144‐153.
   Zhao, R., Natarajan, A., and Srienc, F. 1999. A flow injection flow cytometry system for on‐line monitoring of bioreactors. Biotechnol. Bioeng. 62:609‐617.
Internet Resources
   http://www.beyondlogic.org/serial/serial.htm
  Interfacing the serial/RS232 port. A very informative series of articles by Craig Peacock relevant to the type of serial port programming used in the Plug Flow system.
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