Excitation and Emission Spectra of Common Dyes

Howard M. Shapiro1

1 null, West Newton, Massachusetts
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.19
DOI:  10.1002/0471142956.cy0119s26
Online Posting Date:  November, 2004
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Abstract

This unit tabulates fluorescence excitation and emission spectra of a number of fluorescent probes and labels commonly used in cytometry. This information facilitates selection of probes, light sources, and filters to be used in multicolor experiments.

Keywords: emission; excitation; fluorescence; fluorescent dyes; spectra

     
 
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Table of Contents

  • Fluorescence Basics
  • Fluorescent Probes and Labels Evolve with Cytometers
  • Probes and Spectra
  • Low‐Molecular‐Weight Labels
  • Phycobiliprotein and Tandem Conjugate Labels
  • Nucleic Acid Dyes
  • Fluorescent Reporter Proteins
  • Indo‐1: A Probe of Calcium Concentration
  • Something Completely Different: Quantum Dots
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

   Haugland, R.P. 2002. Handbook of Fluorescent Probes and Research Products, 9th ed. pp. 966. Molecular Probes, Eugene, Oreg.
   Shapiro, H.M. 2003. Practical Flow Cytometry, 4th ed. pp. 681. Wiley‐Liss, New York.
Internet Resources
  http://www.probes.com
  Location for the online version of the Handbook of Fluorescent Probes and Research Products.
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