Fundamentals of Acoustic Cytometry

Michael Ward1, Patrick Turner2, Marc DeJohn3, Gregory Kaduchak1

1 Life Technologies, Eugene, Oregon, 2 Los Alamos Technical Services, Los Alamos, New Mexico, 3 Santa Fe Technical Services, Santa Fe, New Mexico
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.22
DOI:  10.1002/0471142956.cy0122s49
Online Posting Date:  July, 2009
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Abstract

Acoustic cytometry is a new technology that replaces or partly replaces hydrodynamic focusing of cells or particles with focusing derived from acoustic radiation pressure forces. It offers new possibilities for improving current flow cytometry assays and creating new ones. To take full advantage of these possibilities, it is necessary to understand the fundamental benefits and limitations of acoustic focusing as employed in flow cytometry analysis, either as a substitute for hydrodynamic focusing or in combination with it. Curr. Protoc. Cytom. 49:1.22.1‐1.22.12. © 2009 by John Wiley & Sons, Inc.

Keywords: flow cytometry; acoustic focusing; sample preparation

     
 
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Table of Contents

  • Introduction
  • The Acoustic Cytometer
  • Acoustic Force on Particles in a Medium
  • The Acoustic Concentration Effect
  • Summary and Outlook
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
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