Practical Issues in High‐Speed Cell Sorting

Larry W. Arnold1, Joanne Lannigan2

1 The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 2 The University of Virginia, Charlottesville, Virginia
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.24
DOI:  10.1002/0471142956.cy0124s51
Online Posting Date:  January, 2010
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Modern flow cytometric cell sorters are all capable of so‐called “high‐speed sorting.” However, there is confusion about exactly how fast a “high‐speed” cell sorter can sort cells. There are many considerations in achieving the fastest sorting speed, as well as the highest quality sort results—cell recovery, purity, and functionality. This requires the same considerations required for “slow‐speed sorting”; however, a more precise implementation is required for high‐speed sorting. The modern cell sorters enable high‐speed sorting because of advances in high‐speed electronics and data processing. We discuss the practical considerations of high‐speed sorting in terms of the theory and practical aspects of the mechanical and software components of sorting, statistics of sorting, cell preparation and viability, instrument setup, sort strategies, and biosafety. Curr. Protoc. Cytom. 51:1.24.1‐1.24.30. © 2010 by John Wiley & Sons, Inc.

Keywords: flow cytometry; sorting; FACS

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Table of Contents

  • Introduction
  • What is High‐Speed Sorting?
  • Understanding High‐Speed Sorting
  • Practical Aspects of High‐Speed Sorting
  • Safety
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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