Evaluation and Purchase of an Analytical Flow Cytometer: Some of the Numerous Factors to Consider

Robert M. Zucker1, Nancy C. Fisher2

1 U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Toxicology Assessment Division, Research Triangle Park, North Carolina, 2 Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.28
DOI:  10.1002/0471142956.cy0128s63
Online Posting Date:  January, 2013
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Abstract

When purchasing a flow cytometer, the decision of which brand, model, specifications, and accessories may be challenging. The decisions should initially be guided by the specific applications intended for the instrument. However, many other factors need to be considered, which include hardware, software, quality assurance, support, service, and price and recommendations from colleagues. These issues are discussed to help guide the purchasing process. Curr. Protoc. Cytom. 63:1.28.1‐1.28.11. © 2013 by John Wiley & Sons, Inc.

Keywords: flow cytometer; fluorescence; lasers; quality assurance; PMTs

     
 
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Table of Contents

  • Introduction
  • Applications
  • Hardware
  • Software
  • Quality Assurance (QA)
  • Service, Support, and Company
  • Maintenance/Cleanup Protocol
  • Price
  • Recommendation from Colleagues
  • Summary and Conclusions
  • Disclaimer
  • Resources Listed
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Ortyn, W.E., Hall, B.E., George, T.C., Frost, K., Basiji, D.A., Perry, D.J., Zimmerman, C.A., Coder, D., and Morrissey, P.J. 2006. Sensitivity measurement and compensation in spectral imaging. Cytometry A 69:852‐862.
   Shapiro, H.M. 2003. Practical Flow Cytometry, Vol.4. Wiley Liss, New York.
   Zucker, R.M. 2008. Flow Cytometry Quality Assurance in Standardization and Quality Assurance in Fluorescence Measurements 2. Bioanalytical and Biomedical Applications. Springer Series on Fluorescence Methods and Applications (U. Resch‐Genger, ed.; O.S. Wolfbeis, series ed.), pp. 343‐370. Springer‐Verlag, Berlin, Heidelberg.
   Zucker, R.M., Elstein, K.H., Gershey, E.L., and Massaro, E.J. 1990. Increasing sensitivity of the ortho analytical cytofluorograph by modifying the fluid system. Cytometry 11:848‐851.
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