Contrast Enhancement in Light Microscopy

H. Ernst Keller1, Simon Watkins2

1 Carl Zeiss, Inc., Thornwood, New York, 2 Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 2.1
DOI:  10.1002/0471142956.cy0201s63
Online Posting Date:  January, 2013
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Abstract

The optical microscope is a fundamental component of an image cytometry system. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement. Curr. Protoc. Cytom. 63:2.1.1‐2.1.9. © 2013 by John Wiley & Sons, Inc.

Keywords: imaging and microscopy; light microscopy; instrumentation

     
 
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Table of Contents

  • Introduction
  • Köhler Illumination
  • Other Microscopy Techniques
  • Figures
     
 
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Materials

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Figures

Literature Cited

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