Light Microscopy Digital Imaging

James Joubert1, Deepak Sharma1

1 Photometrics, Tucson, Arizona
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 2.3
DOI:  10.1002/0471142956.cy0203s58
Online Posting Date:  October, 2011
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

This unit presents an overview of digital imaging hardware used in light microscopy. CMOS, CCD, and EMCCDs are the primary sensors used. The strengths and weaknesses of each define the primary applications for these sensors. Sensor architecture and formats are also reviewed. Color camera design strategies and sensor window cleaning are also described in the unit. Curr. Protoc. Cytom. 58:2.3.1‐2.3.11. © 2011 by John Wiley & Sons, Inc.

Keywords: microscopy; digital; imaging

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • History of Microscopy Image Capture
  • Solid‐State Sensors
  • Spectral Sensitivity of Sensors
  • Camera Noise
  • Coupling Digital Cameras to Microscopes
  • Color Imaging
  • Camera and Sensor Characteristics
  • Modes of Image Capture
  • Microscope Optimization for Digital Imaging
  • Care and Maintenance
  • Key References
  • Figures
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Key References
   Inoué, S. and Spring, K.R. 1997. Video Microscopy: The Fundamentals. Kluwer Academic/Plenum Publications.
   Janesick, J.R. 2001. Scientific Charge‐Coupled Devices. SPIE Press Monograph, Vol. PM83.
   Kubacki, E. 2006. The dirt on cleaning optics. Photonics Spectra 40:57‐59.
   Murphy, D.B. 2001. Fundamentals of Light Microscopy and Electronic Imaging. Wiley‐Liss, New York.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library