Microscope Alignment

Marc M. Friedman1, Mortimer Abramowitz2

1 Accumed International, Inc., Chicago, Illinois, 2 Olympus America Inc., Mellville, New York
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 2.7
DOI:  10.1002/0471142956.cy0207s02
Online Posting Date:  May, 2001
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Abstract

In order to obtain good images using any microscopic imaging modalities, it is necessary to understand the microscope thoroughly and set up properly. This unit should be required reading for all students and staff using a light microscope. It clearly defines the types of illumination used in microscopes and details the correct set up and operation.

Keywords: light microscopes; Köhler illumination; microscope alignment.

     
 
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Table of Contents

  • The Image‐Forming System
  • Eyepiece Adjustment
  • The Illumination System
  • Maintenance of Optical Glass Surfaces
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Literature Cited

Literature Cited
   Abramowitz, M. 1985. Microscope Basics and Beyond. Olympus Corporation, New York.
   Abramowitz, M. 1987. Contrast Methods in Microscopy: Transmitted Light. Olympus Corporation, New York.
   Abramowitz, M. 1990. Reflected Light Microscopy: An Overview. Olympus Corporation, New York.
   Abramowitz, M. 1993. Fluorescence Microscopy: The Essentials. Olympus America, New York.
   Abramowitz, M. 1994. Optics: A Primer. Olympus America, New York.
   Bradbury, S. 1984. An Introduction to the Optical Microscope. Royal Microscopical Society, Microscopy Handbooks 01. Oxford University Press, Oxford, UK.
   Delly, J.G. 1988. Photography Through the Microscope. Eastman Kodak, Rochester, New York.
   Inoue, S. 1986. Video Microscopy. Plenum Press, New York.
   Olympus, B‐Max, Microscope Guide to Cleaning and Adjustment. Olympus America, New York.
   Leitz, E. 1938. The Microscope and Its Application. Ernst Leitz, Wetzlar, Germany
   Mollring, F.K. 1976. Microscopy From the Very Beginning. Carl Zeiss, Oberkochen, Germany.
   Spencer, M. 1982. Fundamentals of Light Microscopy. IUPAB Biophysics Series, Cambridge University Press, Cambridge, UK.
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