Scanning Laser Cytometry
There are many tasks to which flow cytometers are unsuited, e.g., measurement of attached cells, repeated measurements of a single cell over time, and localization of probes in or on cells. Devices such as confocal microscopes and microscope‐based imaging systems allow high‐resolution analysis and provide flexibility, but typically have low sample throughput and often require a high level of interaction with a technically sophisticated operator. Scanning laser cytometers, which can make low‐resolution, multiparameter optical measurements of light scattering and fluorescence similar to those of flow cytometers, provide reasonably high sample throughput and may, depending on the purpose for which they are designed, either allow for considerable flexibility and operator interaction or permit analysis of large numbers of samples without an operator in attendance. This unit presents an overview of sanning laser cytometers, including such topics as the operating principles, data analysis, and commercially available systems and their applications.
Keywords: scanning laser cytometer; Acumen Explorer HTS; CompuCyte Laser Scanning Cytometer; IMAGN 2000; ChemScan RDI
Figure 2.10.1 Schematic of the optics of the LSC laser scanning cytometer (courtesy of CompuCyte Corporation).
Figure 2.10.2 Schematic of the optics of the IMAGN 2000 volumetric capillary cytometer (courtesy of Biometric Imaging).
Figure 2.10.4 Digitized image of propidium‐stained lymphocyte nuclei produced by the LSC, showing threshold, integration, and background contours (see text). Courtesy of CompuCyte Corporation.
Figure 2.10.5 Identification of micronuclei in cells. See text for details. Courtesy of CompuCyte Corporation.
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