Quality Control in Phenotypic Analysis by Flow Cytometry

Anne A. Hurley1

1 Comprehensive Cytometric Consulting, Ballwin, Missouri
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 6.1
DOI:  10.1002/0471142956.cy0601s00
Online Posting Date:  May, 2001
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Abstract

This unit proposes a specific daily quality control program to ensure instrument reliability and accurate results within current constraints.

     
 
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Table of Contents

  • Introduction
  • Steps for Quality Control in Immunophenotyping
  • Antibody Selection
  • Quantitative Flow Cytometry
  • Conclusion
  • Literature Cited
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Bachner, P. 1996. Quality assurance of the analytic process: Pre‐ and postanalytic variation. Clin. Lab. Med. Vol. 6.
   Duckworth, J.K. 1987. Laboratory licensure and accreditation. In Laboratory Quality Assurance (P.J. Howanitz and J.H. Howanitz, eds.). McGraw‐Hill, New York.
   Poon, R., Johnson, R., Stanton, T., Fernandez‐Repollet, E., and Schwartz, A. 1995. Calibration of fluorescence intensity microbead standards and assignment of effective F/P ratios to the FITC‐conjugated secondary antibody used in the Vth International Leukocyte Typing Workshop. In Leukocyte Typing V pp. 13‐16. Oxford University Press, Oxford.
   Schwartz, A., Fernandez‐Repollett, E., Vogt, R., and Gratama, J. 1996. Standardizing flow cytometry: Construction of a standardized fluorescence calibration plot using matching spectral calibrators. Cytometry 26:22‐31.
   Stelzer, G. T., Marti, G., Hurley, A. A., McCoy, P., Lovett, E. J., and Schwartz, A.. Standardization of technical components of leukemia/lymphoma analysis by flow cytometric immunophenotyping. Submitted for publication.
   Zagursky, R. J., Sharp, D., Solomon, K., and Schwartz, A. 1995. Quantitation of cellular receptors by a new immunocytochemical flow cytometry technique. Biotechniques. 18:3.
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