Data File Standard for Flow Cytometry, FCS 3.0

Larry Seamer1

1 University of New Mexico, Albuquerque, New Mexico
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.2
DOI:  10.1002/0471142956.cy1002s00
Online Posting Date:  May, 2001
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Abstract

This unit describes the data file format FCS 3.0 developed to permit the sharing of data among laboratories and to provide some uniformity in data formats from various instruments.

     
 
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Table of Contents

  • Background
  • Recent Developments
  • File Organization
  • HEADER
  • TEXT
  • DATA
  • ANALYSIS
  • Major New Features in FCS 3.0
  • Summary
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Dean, P.N., Bagwell, C.B., Lindmo, T., Murphy, R.F., and Salzman, G.C. 1990. Data file standard for flow cytometry. Cytometry 11:323‐332.
   Murphy, R.F. and Chused, T.M. 1984. A proposal for a flow cytometric data file standard. Cytometry 5:553‐555.
   Press, W.H., Teukolsky, S.A., Vetterling, W.T., and Flannery, B.P. 1992. Numerical Recipes in C., 2nd ed. Cambridge University Press, Cambridge, UK.
   Redelman, D. and Coder, D.M. 1994. Cell subset (CS) parameter to record the identities of individual cells in flow cytometric data. Cytometry 18:95‐102.
   Unicode Consortium. 1991. The UNICODE Standard, Version 1.0, vol. 1. Addison‐Wesley Publishing, Reading, MA.
Internet Resources
   http://nucleus.immunol.washington.edu/ISAC.html
  Homepage of the International Society for Analytical Cytology (ISAC), with link available to a full listing of FCS 3.0.
   http://journals.wiley.com/cytometry
  Homepage of the John Wiley & Sons, Inc., journal Cytometry, with link available to a full listing of FCS 3.0.
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