Listmode Data Processing

James F. Leary1

1 University of Texas Medical Branch, Galveston, Texas
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.3
DOI:  10.1002/0471142956.cy1003s00
Online Posting Date:  May, 2001
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Abstract

Most flow cytometry data are acquired and stored in listmode format, whereby all measurements on each cell are stored separately in a list, rather than in histograms. This unit discusses the desirability of using listmode format and techniques for handling such data.

     
 
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Table of Contents

  • Background
  • Data File Structures
  • Literature Cited
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Bagwell, C.B. 1993. Theoretical aspects of flow cytometry data analysis. In Clinical Flow Cytometry: Principles and Application (K.D. Bauer, R.E. Duque, and T.V. Shankey, eds) pp. 41‐61. Williams & Wilkins, Baltimore.
   Dean, P.N. 1990. Data processing. In Flow Cytometry and Sorting, 2nd ed. (M.R. Melamed, T. Lindmo, and M.L. Mendelsohn, eds.) pp. 415‐444. Wiley‐Liss, New York.
   Dean, P.N., Bagwell, C.B., Lindmo, T., Murphy, R.F., and Salzman, G.C. 1990. Introduction to flow cytometry data file standard. Cytometry 11:321‐322.
   Jolliffe, I.T. 1986. Principal Component Analysis. Springer‐Verlag, New York.
   Murphy, R.F. and Chused, T.M. 1984. A proposal for a flow cytometric data file standard. Cytometry 5:553‐555.
   Watson, J.V. 1987. Time, a quality‐control parameter in flow cytometry. Cytometry 8:646‐649.
   Watson, J.V. 1992. Flow Cytometry Data Analysis: Basic Concepts and Statistics. Cambridge University Press, Cambridge, U.K.
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