Data Analysis Through Modeling

C. Bruce Bagwell1

1 Verity Software House, Topsham, Maine
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.7
DOI:  10.1002/0471142956.cy1007s01
Online Posting Date:  May, 2001
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Flow cytometers collect data in an attempt to understand some aspect of a biological system. Mathematical models are developed to extract relevant population features from the data, to help users attain their goals. This unit examines what models are and how they are routinely used. Keywords: flow cytometry; listmode data; data analysis; modeling Flow cytometers collect data in an attempt to understand some aspect of a biological system

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Table of Contents

  • A Modeling Story
  • Least‐Squares Analysis
  • Specific Models for Cytometry
  • Steps in Creating a Model
  • Literature Cited
  • Figures
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Literature Cited

Literature Cited
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   Badish, L. 1972. The completeness of nineteenth‐century science. Isis 63:48‐58.
   Bagwell, C.B. 1979. Theory and application of DNA histogram analysis. Ph.D. dissertation, University of Miami, Fla.
   Bagwell, C.B. 1981a. PARA2 Program. Coulter Cytometry, Hialeah, Fla.
   Bagwell, C.B. 1981b. IMMUNO program, EASY2 Software. Coulter Cytometry, Hialeah, Fla.
   Bagwell, C.B. 1993. Theoretical aspects of flow cytometry data analysis. In Clinical Flow Cytometry (K.D. Bauer, R.E. Duque, T.V. Shankey, eds.) pp. 41‐61. Williams & Wilkins, Baltimore.
   Bagwell, C.B. 1996. A journey through flow cytometric immunofluorescence analyses—finding accurate and robust algorithms that estimate positive fraction distributions. Clin. Immunol. Newsletter 16:33‐37.
   Bagwell, C.B., Mayo, S.W., Whetstone, S.D., Hitchcox, S.A., Baker, D.R., Herbert, D.J., Weaver, D.L., Jones, M.A., and Lovett, E.J. III. 1991. DNA histogram debris theory and compensation. Cytometry 12:107‐118.
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   Shankey, T.V., Rabinovitch, P.S., Bagwell, B., Bauer, K.D., Duque, R.E., Hedley, D.W., Mayall, B.H., and Wheeless, L. 1993. Guidelines for implementation of clinical DNA cytometry. Cytometry 14:472‐477.
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