Multivariate Analysis

Michael V. Boland1, Robert F. Murphy1

1 Carnegie Mellon University, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.8
DOI:  10.1002/0471142956.cy1008s03
Online Posting Date:  May, 2001
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Abstract

One of the goals of Current Protocols is to provide information from the very basic level to the very advanced. This unit is an excellent example of providing explanation and application for a complex area. Building upon several other units on data analysis, it provides a detailed explanation of how multivariate analytical techniques are applied to flow cytometry data. The unit deals with the covariance matrix, principal component analysis, and cluster analysis and provides sources for software as well as example data files. This material will be particularly valuable to the graduate student trying to obtain a good understanding of statistical methods in flow cytometry, as well as to the experienced investigator who uses these tools on a regular basis without really understanding how they operate. Keywords: principal components analysis; cluster analysis; FCS file format One of the goals of Current Protocols is to provide information from the very basic level to the very advanced

     
 
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Table of Contents

  • Background Information
  • Principal Components Analysis
  • K‐Means Cluster Analysis
  • Conversion of FCS Files to ASCII Text Files
  • Conversion of ASCII Text Files to FCS Files
  • Importing S‐Plus Data Objects
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Bakker Schut, T.C., De Grooth, B.G., and Greve, J. 1993.Cluster analysis of flow cytometric list mode data on a personal computer. Cytometry 14:649‐659.
   Dean, P.N., Bagwell, C.B., Lindmo, T., Murphy, R.F., and Salzman, G.C. 1990. Data file standard for flow cytometry. Cytometry 11:323‐332.
   Murphy, R.F. 1985. Automated identification of subpopulations in flow cytometric list mode data using cluster analysis. Cytometry 6:302‐309.
   Murphy, R.F. and Chused, T.M. 1984. A proposal for a flow cytometric data file standard. Cytometry 5:553‐555.
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