Image Processing and 2‐D Morphometry

John Turek1

1 Purdue University, West Lafayette, Indiana
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.11
DOI:  10.1002/0471142956.cy1011s13
Online Posting Date:  May, 2001
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Abstract

Image analysis and image processing are becoming commonplace operations in the cytometry environment. This very practical and useful unit describes the basics of image processing, including image enhancement, feature identification, and classification and segmentation. Written by an author with strong background in electron microscopy and image analysis, the unit is well furnished with figures and examples. Anyone needing a start in doing image analysis will find this very easy and useful reading, with suitable references for those wanting to move a step further.

     
 
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Table of Contents

  • Basics of Digital Images
  • Image Processing and Enhancement
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

   Gittes, F. 1990. Estimating mean particle volume and number from random sections by sampling profile boundaries. J. Microsc. 158:1‐18.
   Gundersen, H.J.G. 1988. The nucleator. J. Microsc. 151:3‐21.
   Gundersen, H.J.G. and Jensen, E.B. 1987. The efficiency of systematic sampling in stereology and its prediction. J. Microsc. 147:229‐263.
   Gundersen, H.J.G., Bagger, P., Bendtsen, T.F., Evans, S.M., Korbo, L., Marcussen, N., Moller, A., Nielsen, K., Nyengaard, J.R., Pakkenberg, B., Sorensen, F.B., Vesterby, A., and West, M.J. 1988a. The new stereological tools: dissector, fractionator, and point sampled intercepts and their use in pathological research and diagnosis. Acta Pathol. Microbiol. Immunol. Scand. 96:857‐881.
   Gundersen, H.J.G., Bendtsen, T.F., Korbo, L., Marcussen, N., Moller, A., Nielsen, K., Nyengaard, J.R., Pakkenberg, B., Sorensen, F.B., Vesterby, A., and West, M.J. 1988b. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Acta Pathol. Microbiol. Immunol. Scand. 96:379‐394.
   Jensen, E.B. 1991. Recent developments in the stereological analysis of particles. Ann. Inst. Statist. Math. 43:455‐468.
   Weibel, E.R. 1979. Stereological Methods. Practical Methods for Biological Morphometry. Academic Press, London.
Key References
   Jähne, B. 1993. Digital Image Processing: Concepts, Algorithms, and Scientific Applications, 2nd ed. SpringerVerlag, New York.
  General textbook on digital imaging covering more advanced concepts.
   Robinson, J.P. and Turek, J.J. 1999. Microscope image processing and analysis. Encyclopedia of Electronics and Engineering, Vol. 13 9‐21. John Wiley Sons, New York.
  General basic reference for digital imaging. Includes background on confocal and electron microscopy.
   Serra, J. 1993. Image Analysis and Mathematical Morphology. Academic Press, London.
  Foundational text for understanding morphological operations in digital imaging.
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