Dial‐In Flow Cytometry Data Analysis

Francis L. Battye1

1 The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 10.12
DOI:  10.1002/0471142956.cy1012s19
Online Posting Date:  February, 2002
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Abstract

As listmode data files continue to grow larger, access via any kind of network connections becomes more and more trouble because of the enormous traffic generated. The limited speed of transmission via modem makes analysis almost impossible. This unit presents a solution to these problems, one that involves installation at the central storage facility of a small computer program called a Web servlet. Operating in concert with a Web server, the servlet assists the analysis by extracting the display array from the data file and organizing its transmission over the network to a remote client program that creates the data display. The author discusses a recent implementation of this solution and the results for model transmission of two typical data files. The system greatly speeds access to remotely stored data yet retains the flexibility of manipulation expected with local access.

     
 
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Table of Contents

  • Communication Between the Analysis Program and the Servlet
  • Data Compression
  • Computer Software Construction
  • Anticipated Results
  • Current and Future Implications
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   Battye, F.L. 2001. Web servlet‐assisted, dial‐in flow cytometry data analysis. Cytometry 43:143‐149.
   Hamilton, G., Cattell, R., and Fisher, M. 1997. JDBC Database Access with Java: A Tutorial and Annotated Reference. Addison Wesley Longman, Reading, Mass.
   Moore, W.A. and Kautz, R.A. 1986. Data analysis in flow cytometry. In Handbook of Experimental Immunology, 4th edition (D.M. Weir, ed.) Vol. 1, Chpt. 30. Blackwell Scientific, Oxford.
   Olivares, G., Habbersett, R., and Coder, D. 1998. The National Flow Cytometry Resource Cytometry Reference Database. Cytometry Supplement 9:57.
   Seamer, L.C., Bagwell, C.B., Barden, L., Redelman, D., Salzman, G.C., Wood, J.C.S., and Murphy, R.F. 1997. Proposed new data file standard for flow cytometry, version FCS 3.0. Cytometry 28:118‐122.
Internet Resources
   http://www.wehi.edu.au/cytometry/WEASEL.html
  Description of the features and operation of the WEASEL flow cytometry data analysis program.
   http://java.sun.com/nav/whatis/
  A guide to what the Java platform is and how it can be used.
   http://java.sun.com/j2se/
  Source for downloading software development kits and runtime environments for Java on Windows, Solaris, and Linux operating systems.
   http://developer.apple.com/java/
  Source for downloading software development kits and runtime environments for Java on Macintosh OS X or Classic operating systems.
   http://java.sun.com/docs/books/tutorial/
  Sun Microsystems' Java tutorial dealing with all aspects of Java Programming.
   http://java.sun.com/docs/books/tutorial/networking/urls/index.html
  Section of Sun Microsystems' Java Tutorial dealing with the way applications may connect to and read from network sites defined by universal resource locators (URLs).
   http://java.sun.com/docs/books/tutorial/servlets/index.html
  Section of Sun Microsystems' Java Tutorial dealing with servlets.
   http://java.sun.com/products/servlet/archive.html
  Site for downloading the Java servlet development kit.
   http://java.sun.com/products/servlet/industry.html
  Documents the support for Java servlets among many popular Web servers.
   http://jakarta.apache.org/tomcat/
  Description of the Tomcat addition to the Apache Web server that is used as a servlet container.
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