Overview of Flow Cytometry and Microbiology

J. Paul Robinson1

1 Purdue University, West Lafayette, Indiana
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 11.1
DOI:  10.1002/0471142956.cy1101s30
Online Posting Date:  November, 2004
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Although in recent years flow cytometry has become commonplace in hematology and immunology laboratories, application of the technology to microbiology remains largely unrealized. This overview presents the historical background, discusses applications in various areas of the field, and speculates on the directions of future developments. The availability of high‐quality methods should be a prime factor in convincing microbiologists that flow cytometry may have certain advantages over traditional methods and that it does indeed have much to contribute to microbiology.

Keywords: flow cytometry; microbiology; environment; food microbiology; overvie

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Table of Contents

  • Historical Development
  • Applications
  • Future Development
  • Literature Cited
  • Tables
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Literature Cited

Literature Cited
   Allman, R., Manchee, R., and Lloyd, D. 1993. Flow cytometric analysis of heterogeneous bacterial populations. In Flow Cytometry in Microbiology (D. Lloyd, ed.) pp. 27‐47. Springer‐Verlag, New York.
   Amann, R. 1995. Fluorescently labeled, rRNA‐targeted oligonucleotide probes in the study of microbial ecology. Mol. Ecol. 4:543‐554.
   Amann, R.I., Binder, B.J., Olson, R.J., Chisholm, S.W., Devereux, R., and Stahl, D.A. 1990. Combination of 16S rRNA–targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919‐1925.
   Bogosian, G. 1998. Viable but nonculturable, or dead? ASM News 64:547.
   Boye, E., Steen, H.B., and Skarstad, K. 1983. Flow cytometry of bacteria: A promising tool in experimental and clinical microbiology. J. Gen. Microbiol. 129:973‐980.
   Cunningham, A. 1993. Analysis of microalgae and cyanobacteria by flow cytometry. In Flow Cytometry in Microbiology (D. Lloyd, ed.) pp. 131‐142. Springer‐Verlag, New York.
   Edwards, C., Diaper, J.P., Porter, J., and Pickup, R. 1993. Applications of flow cytometry in bacterial ecology. In Flow Cytometry in Microbiology (D. Lloyd, ed.) pp. 121‐129. Springer‐Verlag, New York.
   Hercher, M., Mueller, W., and Shapiro, H.M. 1979. Detection and discrimination of individual viruses by flow cytometry. J. Histochem. Cytochem. 27:350‐352.
   Hutter, K.‐J. and Eipel, H.E. 1979. Microbial determinations by flow cytometry. J. Gen. Microbiol. 113:369‐375.
   Ingram, M., Cleary, T.J., Price, B.J., Price, R.L., and Castro, A. 1982. Rapid detection of Legionella pneumophila by flow cytometry. Cytometry 3:134‐137.
   Salzman, G.C., Griffith, J.K., and Gregg, C.T. 1982. Rapid identification of microorganisms by circular‐intensity differential scattering. Appl. Environ. Microbiol. 44:1081‐1085.
   Shapiro, H.M. 2003. Practical Flow Cytometry, 4th ed. Wiley‐Liss, New York.
   Spencer, R.C. and Lightfooty, N.F. 2001. Preparedness and response to bioterrorism. J. Infect. 43:104‐110.
   Steen, H.B. 1986. Simultaneous separate detection of low angle and large angle light scattering in an arc lamp‐based flow cytometer. Cytometry. 7:445‐449.
   Tarran, G.A. and P.H., Burkill 1993. Flow cytometry at sea. In Flow Cytometry in Microbiology (D. Lloyd, ed.) pp. 143‐158. Springer‐Verlag, New York.
   Troussellier, M., Courties, C., and Vaquer, A. 1993. Recent applications of flow cytometry in aquatic microbial ecology. Biol. Cell 78:111‐121.
   Wallner, G., Erhart, R., and Amann, R. 1995. Flow cytometric analysis of activated sludge with rRNA‐targeted probes. Appl. Environ. Microbiol. 61:1859‐1866.
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