Flow Cytometry and Environmental Microbiology

Jonathan Porter1

1 University of Exeter, Exeter
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 11.2
DOI:  10.1002/0471142956.cy1102s27
Online Posting Date:  February, 2004
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Abstract

This survey unit discusses many of the issues involved for flow cytometry in the field of microbiology, particularly the preparative procedures, which are far more stringent than many other procedures using larger cells. For instance, it is often necessary to filter laboratory agents multiple times to obtain the true particle‐free solutions needed for flow cytometry of microbes. It is difficult enough to recognize bacteria in cell extracts from soil, sediment, or sludge given the background of same‐size particles. This unit provides an excellent overview of a potentially large application area in flow cytometry and is written by one of the most respected scientists in the field.

Keywords: flow cytometry; cell sorting; environmental microbiology; viability; biomass

     
 
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Table of Contents

  • Applying Flow Cytometry and Cell Sorting to Environmental Microbiology
  • Environmental Monitoring of Microorganisms Using Flow Cytometry and Cell Sorting
  • Specific Detection of Microorganisms in Environmental Samples Using Flow Cytometry
  • Viability Assessment of Microorganisms Using Flow Cytometry
  • Linking Identity, Function, and Activity in Single Microbial Cells
  • Discussion and Future Prospects
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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  Dr. Porter wishes to acknowledge the support of the Natural Environment Research Council, Swindon, United Kingdom.
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