Comparative Overview of Flow and Image Cytometry

J. Paul Robinson1

1 Purdue University, West Lafayette, Indiana
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 12.1
DOI:  10.1002/0471142956.cy1201s31
Online Posting Date:  February, 2005
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Abstract

This unit considers the issues of which tool to use—flow cytometry or imaging—and under what conditions. In particular, it compares the advantages and disadvantages of flow and image cytometry and provides examples illustrating the proper choice of each technology. The result is a better understanding of why the two technologies are complementary in many applications. It is clear that many scientists use the tools that are familiar to them, often in preference to the best tool. In cases where very advanced and rather expensive technologies are concerned, this is not surprising. However, there are clearly times when one form of cytometry is definitely superior to another. What then constitute the criteria for a decision when both flow cytometry and imaging are available? This unit addresses some of these concerns.

Keywords: flow cytometry; image cytometry; fluorescence measurement

     
 
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Table of Contents

  • Introduction to Flow Cytometry
  • Introduction to Imaging
  • Introduction to Scanning Laser Cytometry
  • Advantages of Flow Cytometry
  • Advantages of Imaging
  • Comparison of Traditional Imaging and Flow Cytometry
  • Combination Studies with Flow and Imaging
  • Imaging Cytometry
  • Conclusion
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   De Rosa, S.C. and Roederer, M. 2001. Eleven‐color flow cytometry. A powerful tool for elucidation of the complex immune system. Clin. Lab. Med. 21:697‐712.
   Gerstner, A.O.H., Trumpfheller, C., Racz, P., Osmancik, P., Tenner‐Racz, K., and Tárnok, A. 2004. Quantitative histology by multicolor slide‐based cytometry. Cytometry 59A:210‐219.
   Lenz, D., Gerstner, A., Laffers, W., Steinbrecher, M., Bootz, F., and Tárnok, A. 2003. Six and more color immunophenotyping on the slide by Laser Scanning Cytometry (LCS). Proc. SPIE 4962:364‐374.
   Salvioli, S., Ardizzoni, A., Franceschi, C., and Cossarizza, A. 1997. JC‐1, but not DiOC6(3) or rhodamine, is a reliable fluorescent probe to assess delta psi changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411:77‐82.
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