Modern Confocal Microscopy

Bartek Rajwa1

1 Purdue University Cytometry Laboratories, West Lafayette, Indiana
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 12.3
DOI:  10.1002/0471142956.cy1203s31
Online Posting Date:  February, 2005
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Abstract

This unit re‐examines confocal microscopy from a current perspective. It outlines many of the most modern applications of confocal microscopy and the issues surrounding them. The expanding applications of confocal microscopy demand both minor and major modifications of the technology to enhance imaging capabilities for a growing variety of samples. Techniques of interest such as FRET, FLIP, FRAP, and IFRAP are described. The unit includes a discussion on multispectral imaging, potentially the most exciting innovation in the field of biological imaging. The other area that is beginning to have considerable impact on biology, medicine, and pharmacology comprises high‐content screening (HCS) and high‐throughput screening (HTS). This unit also introduces programmable array microscopes (PAMs), which are based upon a technique in which spatial modulators are placed in the imaging plane of a microscope and used to generate patterns of illumination and/or detection.

Keywords: confocal microscopy; multispectral imaging; programmable array microscope; FRET; FRAP

     
 
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Table of Contents

  • Modern Applications of Confocal Microscopy
  • Progress in Confocal Microscopy Hardware
  • Conclusion
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
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