Confocal Imaging of Cell Division

Paul S. Maddox1

1 Department of Pathology and Cell Biology, Université de Montréal, Montreal, Canada
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 12.11
DOI:  10.1002/0471142956.cy1211s43
Online Posting Date:  January, 2008
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Imaging of subcellular events has been key to recent advances in the basic knowledge base as well as drug discovery for disease states such as cancer. In a snowball effect, advances in imaging have spurred new investigations, which have increased the demand for new technologies. The study of cell division serves as an example of this cycle. Here, application of spinning disk confocal technology to the study of cell division is discussed. While these studies have increased understanding of fundamental mechanisms in several mitotic events, new imaging technologies in the future will unlock more secrets of cell biology. Curr. Protocol. Cytom. 43:12.11.1‐12.11.13. © 2008 by John Wiley & Sons, Inc.

Keywords: confocal imaging; live cell imaging; mitosis; cell division; microtubule; kinetochore; chromosome condensation; cytokinesis

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Table of Contents

  • Introduction
  • Spinning Disk Confocal
  • Confocal Imaging of Chromosome Condensation in C. elegans Embryos
  • Confocal Imaging of Spindle Assembly and Chromosome Dynamics
  • Confocal Imaging of Cytokinesis
  • Discussion
  • Acknowledgements
  • Literature Cited
  • Figures
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Literature Cited

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