This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization, immunohistochemistry, and enzyme histochemistry. Curr. Protocol. Cytom. 48:12.15.1‐12.15.7. © 2009 by John Wiley & Sons, Inc.
Keywords: fixation; frozen; cryosections; immunohistochemisty; antibody
Basic Protocol 1: Specimen Preparation and Sectioning
Support Protocol 1: Tissue Fixation and Sucrose Infusion
Support Protocol 2: Perfusion of Adult Mice
Figure 12.15.2 Sectioning procedure. A to E illustrate cryosectioning and correspond to steps 6 to 13 of the ; C to E also illustrate paraffin wax sectioning.