Photoactivation and Imaging of Optical Highlighter Fluorescent Proteins

George H. Patterson1

1 Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 12.23
DOI:  10.1002/0471142956.cy1223s57
Online Posting Date:  July, 2011
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Abstract

A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be “turned on” by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks. Curr. Protoc. Cytom. 57:12.23.1‐12.23.12. © 2011 by John Wiley & Sons, Inc.

Keywords: photoactivatable; fluorescent protein; microscopy

     
 
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Table of Contents

  • Introduction
  • Background
  • Requirements for Highlighting Fluorescent Proteins
  • Optimization Procedures
  • General Photoactivation Experiment
  • Uses of Optical Highlighter Fluorescent Proteins
  • Application of Optical Highlighter Fluorescent Proteins in Cytometry
  • Future Directions of Optical Highlighter Fluorescent Proteinsxsxs
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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