Correlative Fluorescence and Electron Microscopy

Randall T. Schirra1, Peijun Zhang1

1 Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 12.36
DOI:  10.1002/0471142956.cy1236s70
Online Posting Date:  October, 2014
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Abstract

Correlative fluorescence and electron microscopy (CFEM) is a multimodal technique that combines dynamic and localization information from fluorescence methods with ultrastructural data from electron microscopy, to give new information about how cellular components change relative to the spatiotemporal dynamics within their environment. In this review, we will discuss some of the basic techniques and tools of the trade for utilizing this attractive research method, which is becoming a very powerful tool for biology labs. The information obtained from correlative methods has proven to be invaluable in creating consensus between the two types of microscopy, extending the capability of each, and cutting the time and expense associated with using each method separately for comparative analysis. The realization of the advantages of these methods in cell biology has led to rapid improvement in the protocols and has ushered in a new generation of instruments to reach the next level of correlation—integration. Curr. Protoc. Cytom. 70:12.36.1‐12.36.10. © 2014 by John Wiley & Sons, Inc.

Keywords: electron microscopy; fluorescence; light microscopy; correlative; cell biology; imaging; cryoEM; immunolabel; tomography; integrated microscope

     
 
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Table of Contents

  • Introduction
  • Rationale
  • Methods
  • Remarks on the Future of CFEM
  • Acknowledgements
  • Literature Cited
  • Figures
     
 
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Materials

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Literature Cited

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