Common Stock Solutions, Buffers, and Media


Publication Name:  Current Protocols in Cytometry
Unit Number:  Appendix 2A
DOI:  10.1002/0471142956.cya02as09
Online Posting Date:  May, 2001
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Abstract

    Recipes

  • Ammonium chloride lysing solution, 10×

  • Complete DMEM

  • Complete RPMI

  • DTT (DL‐dithiothreitol), 0.1 M

  • EDTA (ethylenediamine tetraacetic acid), 0.5 M, pH 8

  • Ethidium bromide staining solution

  • FBS (fetal bovine serum)

  • Formamide, deionized

  • Gel loading buffer, 6×

  • L‐Glutamine, 0.2 M (100×)

  • HBSS (Hanks' buffered salt solution)

  • PBS (phosphate‐buffered saline)

  • RNase A stock solution (DNase‐free), 2 mg/ml

  • SSC, 20×

  • TAE buffer, 50×

  • TBE buffer, 10×

  • TE buffer

  • Tris⋅Cl, 1 M

  • Trypsin/EDTA solution

     
 
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Table of Contents

         
     
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    Materials

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    Literature Cited

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