Techniques for Bacterial Cell Culture: Media Preparation and Bacteriological Tools

Karen Lech1, Roger Brent1

1 Massachusetts General Hospital and Harvard, Medical School Boston, Massachusetts
Publication Name:  Current Protocols in Cytometry
Unit Number:  Appendix 3E
DOI:  10.1002/0471142956.cya03es11
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

Basic information for the culture of bacteria is presented in this appendix, using Escherichia coli as a representative organism. The techniques and recipes should be readily transferrable. Specifics are given for liquid and solid media, rich and minimal media, stab agar, as well as a description of the tools required to inoculate and culture bacteria.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Media Preparation
  • Minimal Media
  • Rich Media
  • Solid Media
  • Tools
  • Literature Cited
  • Figures
  • Tables
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Foster, T.J. 1983. Plasmid‐determined resistance to antimicrobial drugs and toxic metal ions in bacteria. Microbiol. Rev. 47:361‐409.
   Gottlieb, D. Shaw, P.D. 1967. Antibiotics. I. Mechanism of Action. Springer‐Verlag, New York.
   Lech, K. and Brent, R. 1988. Plating lambda phage to generate plaques. In Current Protocols in Molecular Biology (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, eds.) pp. 1.11.1‐1.11.4. John Wiley & Sons, New York.
   Moazed, D. and Noller, H.F. 1987. Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature 327:389‐394.
   Quertermous, T. 1996. Plating and transferring bacteriophage libraries. In Current Protocols in Molecular Biology (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, eds.) pp.6.1.1‐6.1.4. John Wiley & Sons, New York.
   Raleigh, E.A., Lech, K., and Brent, R. 1989. Selected topics from classical genetics. In Current Protocols in Molecular Biology (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, eds.) pp.1.4.1‐1.4.14. John Wiley & Sons, New York.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library