Growing Bacteria in Liquid Media

Karen Lech1, Roger Brent1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Cytometry
Unit Number:  Appendix 3F
DOI:  10.1002/0471142956.cya03fs12
Online Posting Date:  May, 2001
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Abstract

The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.

     
 
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Table of Contents

  • Basic Protocol 1: Growing an Overnight Culture
  • Growing Larger Cultures
  • Basic Protocol 2: Monitoring Growth
  • Figures
     
 
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Materials

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Figures

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Literature Cited

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