Growing Bacteria on Solid Media

Karen Lech1, Roger Brent1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Cytometry
Unit Number:  Appendix 3G
DOI:  10.1002/0471142956.cya03gs12
Online Posting Date:  May, 2001
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Abstract

Detailed protocols are provided for titering and isolating bacterial colonies by serial dilutions, or alternatively by streaking or spreading a plate. Support protocols describe replica plating as well as methods for storing strains as agar stabs or frozen glycerol stocks.

     
 
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Table of Contents

  • Basic Protocol 1: Titering and Isolating Bacterial Colonies by Serial Dilutions
  • Basic Protocol 2: Isolating Single Colonies by Streaking a Plate
  • Basic Protocol 3: Isolating Single Colonies by Spreading a Plate
  • Support Protocol 1: Replica Plating
  • Support Protocol 2: Strain Storage and Revival
  • Frozen Stocks
  • Literature Cited
  • Figures
     
 
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Materials

Basic Protocol 1: Titering and Isolating Bacterial Colonies by Serial Dilutions

  Materials
  • LB medium ( appendix 3E)
  • LB plates ( appendix 3E)
  • Sterile 16‐ or 18‐mm‐diameter culture tubes
  • Bacterial suspension to be titered
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Figures

Videos

Literature Cited

Literature Cited
   Adams, J.N. 1965. Automotive pistons for use as bases in velveteen replication. J. Bacteriol. 89:1627.
   Raleigh, E.A., Lech, K., and Brent, R. 1989. Selected topics from classical bacterial genetics. In Current Protocols in Molecular Biology (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, eds.) pp. 1.4.1‐1.4.13. John Wiley & Sons, New York.
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